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plenti cas9 gfp plasmid  (Addgene inc)


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    Addgene inc plenti cas9 gfp plasmid
    Plenti Cas9 Gfp Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti cas9 gfp plasmid/product/Addgene inc
    Average 93 stars, based on 39 article reviews
    plenti cas9 gfp plasmid - by Bioz Stars, 2026-06
    93/100 stars

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    ( A ) Schematic representation of control (Sox2P_WT-CBS) and edited (Sox2P_ΔCBS-in-SCR & Sox2P_ΔCBS-DS-SCR) cell lines containing the Sox2P reporter in LP-116 kb. Blue rectangle: Sox2P reporter; red: Sox2 ::mCherry; green: Sox2 ::eGFP; orange: SCR; position and orientation of grey triangles indicate endogenous CBS; pink triangles indicate <t>CRISPR/Cas9</t> deleted CBSs. (B) Endogenous Sox2 levels ( Sox2 ::eGFP & Sox2 ::mCherry) and Sox2P reporter expression ( Sox2 ::mTurq) of control (non-fluorescent control and CBS-WT control) and CBS deletion cell lines (Sox2P_ΔCBS-in-SCR & Sox2P_ΔCBS-DS-SCR) containing the Sox2P reporter in LP-116 kb measured by FACS. (C) Top: Schematic of hopping the Sox2P reporter; Bottom: Sox2P reporter and Sox2 ::eGFP FACS measurements of SB transfected cells. Reporter expression is divided into six gates (P1-P6) and cell pools were sorted for each gate. Percentage indicates the frequency of cells occurring per population. (D) Distribution of mapped integrations per cell line (CBS-WT; Sox2P_ΔCBS-in-SCR, Sox2P_ΔCBS-DS-SCR) and sorted population (P1-P6). Each dot represents a mapped integration. The number of integrations plotted per population is indicated by n. Ctrl integrations refer to the mapping of a large pool of unsorted (hopped) cells. Grey vertical line: position of LP-116 kb; red vertical square: position of Sox2::mCherry ; orange vertical square: position of the SCR. (E) Expression score calculate of Sox2P integrations mobilized from LP-116 kb in CBS-WT (ctrl) and ΔCBS-in-SCR in (D), smoothened using a 20kb window shifted by 1kb steps and only plotted if at least three integrations per window are present. Shaded region indicates 95% CI. Grey vertical dotted lines and triangles indicate the position and orientation of CBSs. (F) Same as (E), except the comparison with cell line ΔCBS-DS-SCR is displayed.
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    ( A ) Schematic representation of control (Sox2P_WT-CBS) and edited (Sox2P_ΔCBS-in-SCR & Sox2P_ΔCBS-DS-SCR) cell lines containing the Sox2P reporter in LP-116 kb. Blue rectangle: Sox2P reporter; red: Sox2 ::mCherry; green: Sox2 ::eGFP; orange: SCR; position and orientation of grey triangles indicate endogenous CBS; pink triangles indicate <t>CRISPR/Cas9</t> deleted CBSs. (B) Endogenous Sox2 levels ( Sox2 ::eGFP & Sox2 ::mCherry) and Sox2P reporter expression ( Sox2 ::mTurq) of control (non-fluorescent control and CBS-WT control) and CBS deletion cell lines (Sox2P_ΔCBS-in-SCR & Sox2P_ΔCBS-DS-SCR) containing the Sox2P reporter in LP-116 kb measured by FACS. (C) Top: Schematic of hopping the Sox2P reporter; Bottom: Sox2P reporter and Sox2 ::eGFP FACS measurements of SB transfected cells. Reporter expression is divided into six gates (P1-P6) and cell pools were sorted for each gate. Percentage indicates the frequency of cells occurring per population. (D) Distribution of mapped integrations per cell line (CBS-WT; Sox2P_ΔCBS-in-SCR, Sox2P_ΔCBS-DS-SCR) and sorted population (P1-P6). Each dot represents a mapped integration. The number of integrations plotted per population is indicated by n. Ctrl integrations refer to the mapping of a large pool of unsorted (hopped) cells. Grey vertical line: position of LP-116 kb; red vertical square: position of Sox2::mCherry ; orange vertical square: position of the SCR. (E) Expression score calculate of Sox2P integrations mobilized from LP-116 kb in CBS-WT (ctrl) and ΔCBS-in-SCR in (D), smoothened using a 20kb window shifted by 1kb steps and only plotted if at least three integrations per window are present. Shaded region indicates 95% CI. Grey vertical dotted lines and triangles indicate the position and orientation of CBSs. (F) Same as (E), except the comparison with cell line ΔCBS-DS-SCR is displayed.
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    ( A ) Schematic representation of control (Sox2P_WT-CBS) and edited (Sox2P_ΔCBS-in-SCR & Sox2P_ΔCBS-DS-SCR) cell lines containing the Sox2P reporter in LP-116 kb. Blue rectangle: Sox2P reporter; red: Sox2 ::mCherry; green: Sox2 ::eGFP; orange: SCR; position and orientation of grey triangles indicate endogenous CBS; pink triangles indicate CRISPR/Cas9 deleted CBSs. (B) Endogenous Sox2 levels ( Sox2 ::eGFP & Sox2 ::mCherry) and Sox2P reporter expression ( Sox2 ::mTurq) of control (non-fluorescent control and CBS-WT control) and CBS deletion cell lines (Sox2P_ΔCBS-in-SCR & Sox2P_ΔCBS-DS-SCR) containing the Sox2P reporter in LP-116 kb measured by FACS. (C) Top: Schematic of hopping the Sox2P reporter; Bottom: Sox2P reporter and Sox2 ::eGFP FACS measurements of SB transfected cells. Reporter expression is divided into six gates (P1-P6) and cell pools were sorted for each gate. Percentage indicates the frequency of cells occurring per population. (D) Distribution of mapped integrations per cell line (CBS-WT; Sox2P_ΔCBS-in-SCR, Sox2P_ΔCBS-DS-SCR) and sorted population (P1-P6). Each dot represents a mapped integration. The number of integrations plotted per population is indicated by n. Ctrl integrations refer to the mapping of a large pool of unsorted (hopped) cells. Grey vertical line: position of LP-116 kb; red vertical square: position of Sox2::mCherry ; orange vertical square: position of the SCR. (E) Expression score calculate of Sox2P integrations mobilized from LP-116 kb in CBS-WT (ctrl) and ΔCBS-in-SCR in (D), smoothened using a 20kb window shifted by 1kb steps and only plotted if at least three integrations per window are present. Shaded region indicates 95% CI. Grey vertical dotted lines and triangles indicate the position and orientation of CBSs. (F) Same as (E), except the comparison with cell line ΔCBS-DS-SCR is displayed.

    Journal: bioRxiv

    Article Title: Effects of CTCF on the regulatory landscape of the mouse Sox2 locus

    doi: 10.64898/2026.03.16.711995

    Figure Lengend Snippet: ( A ) Schematic representation of control (Sox2P_WT-CBS) and edited (Sox2P_ΔCBS-in-SCR & Sox2P_ΔCBS-DS-SCR) cell lines containing the Sox2P reporter in LP-116 kb. Blue rectangle: Sox2P reporter; red: Sox2 ::mCherry; green: Sox2 ::eGFP; orange: SCR; position and orientation of grey triangles indicate endogenous CBS; pink triangles indicate CRISPR/Cas9 deleted CBSs. (B) Endogenous Sox2 levels ( Sox2 ::eGFP & Sox2 ::mCherry) and Sox2P reporter expression ( Sox2 ::mTurq) of control (non-fluorescent control and CBS-WT control) and CBS deletion cell lines (Sox2P_ΔCBS-in-SCR & Sox2P_ΔCBS-DS-SCR) containing the Sox2P reporter in LP-116 kb measured by FACS. (C) Top: Schematic of hopping the Sox2P reporter; Bottom: Sox2P reporter and Sox2 ::eGFP FACS measurements of SB transfected cells. Reporter expression is divided into six gates (P1-P6) and cell pools were sorted for each gate. Percentage indicates the frequency of cells occurring per population. (D) Distribution of mapped integrations per cell line (CBS-WT; Sox2P_ΔCBS-in-SCR, Sox2P_ΔCBS-DS-SCR) and sorted population (P1-P6). Each dot represents a mapped integration. The number of integrations plotted per population is indicated by n. Ctrl integrations refer to the mapping of a large pool of unsorted (hopped) cells. Grey vertical line: position of LP-116 kb; red vertical square: position of Sox2::mCherry ; orange vertical square: position of the SCR. (E) Expression score calculate of Sox2P integrations mobilized from LP-116 kb in CBS-WT (ctrl) and ΔCBS-in-SCR in (D), smoothened using a 20kb window shifted by 1kb steps and only plotted if at least three integrations per window are present. Shaded region indicates 95% CI. Grey vertical dotted lines and triangles indicate the position and orientation of CBSs. (F) Same as (E), except the comparison with cell line ΔCBS-DS-SCR is displayed.

    Article Snippet: One guide per region was selected (pCM036 for ‘ΔCBS-in-SCR’ and pCM032 for ‘ΔCBS-DS-SCR) and 0.5 million cells were transfected with 1 ug Cas9 plasmid (pX458, Addgene 48138) and 1.5 ug gRNA plasmid using 7.5 ul lipofectamine 2000.

    Techniques: Control, CRISPR, Expressing, Transfection, Comparison